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PCR technology : current innovations / edited by Thomas Weissenteiner, Hugh G. Griffin, and Annette Griffin.

Contributor(s): Publication details: Boca Raton, Fla. : CRC Press, c2004.Edition: 2nd. edDescription: 392 p. : ill. ; 26 cmISBN:
  • 0849311845 (pbk.)
Subject(s): DDC classification:
  • 572.8636 PCR
Contents:
Section I : General Considerations. : Template Isolation and Preparation. - Ch. 1 Extraction and Amplification of Ancient DNA / Anne C. Stone. - Ch. 2 Long PCR from Damaged DNA / Bernard Fromenty, Philippe Schmitt, Dominique Pessayre. - Ch. 3 Quantitative mRNA Analysis in Small Cell Samples by RT-PCR and Flow Cytometry / Veronika Stemme, Sten Stemme. - Ch. 4 Microdissection of Single or Small Numbers of Cells for Analyses of DNA and RNA by PCR / Rose G. Mage, Robert F. Bonner, Harold Obiakor. - Ch. 5 Pre-PCR Processing Strategies / Peter Radstrom, Maria Lovenklev, Petra Wolffs, Charlotta Lofstrom, Rickard Knutsson. - Ch. 6 Novel PCR-Enhancing Compounds and Their Modes of Action / Raj Chakrabarti. - Ch. 7 Optimizing PCR with the Aid of Experimental Design / Thomas Weissensteiner. - Ch. 8 Economic Fluorescent Labeling of PCR Products for Microsatellite- and Single-Stranded Conformation Polymorphism (SSCP) Analysis / Markus Schuelke. - Ch. 9 Universal Restriction Site-Free Cloning Method Using Chimeric Primers / Guo Jun Chen. - Ch. 10 High-Throughput Genotyping and the PCR / Kalim U. Mir, Jiannis Ragoussis . - Ch. 11 High-Throughput Genetic Analysis through Multiplexed PCR and Multicapillary Electrophoresis / John M. Butler, Peter M. Vallone. - Ch. 12 The GOOD Assay: A Purification-Free Assay for Genotyping by MALDI Mass Spectrometry / Jorg Tost, Ramon Kucharzak, Doris Lechner, Ivo Glynne Gut. - Ch. 13 Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension (APEX) / Maido Remm, Ants Kurg, Andres Metspalu. - Ch. 14 Surface Plasmon Resonance Based Biosensor Technology for Real-Time Detection of PCR Products / Giordana Feriotto, Roberto Gambari. - Ch. 15 Fluorescent Amplified Fragment Length Polymorphism (FAFLP) Genotyping / Catherine Arnold. - Ch. 16 Global Analysis of DNA Allelic Variations (GADAV) by Specific Enrichment of Mismatches and Selective Amplification of Heterohybrids / Xinghua Pan, Sherman M. Weissman. - Ch. 17 Quantitative DNA-Methylation Analysis by the Bisulfite Conversion Method / Osman El-Maarri, Manfred Kuepper, Johannes Oldenburg, Jorn Walter. - Ch. 18 Pyrosequencing Technology / Pal Nyren, Tommy Nordstrom, Baback Gharizadeh. - Ch. 19 Octamer-Primed Cycle Sequencing / Anelia I. Kraltcheva, Susan H. Hardin. - Ch. 20 In Situ Amplification of cDNA / Gerard J. Nuovo. - Ch. 21 Detection of a Single-Copy Sequence on Human Metaphase by Cyclic PRINS / Kirsten Mennicke, Sven Opitz, Eberhard Schwinger. - Ch. 22 Meaningful Quantification of mRNA Using Real-Time PCR / Stephen A. Bustin. - Ch. 23 Absolute Quantification of Specific Nucleic Acids by RT-PCR Using a Nonlinear Mathematical Model for Data Analysis / Huong L. Vu, Serge Troubetzkoy, Vu Q. Nguyen, Michael W. Russell, Jiri Mestecky. - Ch. 24 Single-Molecule PCR - Basic Protocols and Applications / Paul H. Dear. - Ch. 25 Gene Expression and Discovery: Application of a Genome-Wide Approach / Timothy A. Lohret, Karen M. Kelly. - Ch. 26 The Detection of Differential Gene Expression Changes Using SAGE (Serial Analysis of Gene Expression) / Jill Powell. - Ch. 27 Applications of Differential Display in Infectious Diseases / Irene Bosch, Rajas V. Warke, Marcia F. Fournier, David Magee, Heather Melichar. - Ch. 28 Restriction Endonucleolytic Analysis of Differentially Expressed Sequences: READS / Yerramilli V. B. K. Subrahmanyam, Sherman M. Weissman. - Ch. 29 Suppression Subtractive Hybridization PCR / Wan Ji, Li Cai. - Ch. 30 PCR-Based Techniques for Cloning an Unknown DNA Fragment Adjacent to a Known Integrant / Eric Ka-Wai Hui, Szecheng J. Lo. - Ch. 31 Chromosome Walking by Inverse PCR / Jorge A. Garces, Selwyn A. Williams, Ray H. Gavin. - Ch. 32 Long Universal Fast Walking / Kyl V. Myrick. - Ch. 33 Cloning of Unknown Virus Sequences by DNase Treatment and Sequence-Independent Single Primer Amplification / Tobias Allander, Suzanne U. Emerson, Robert H. Purcell, Jens Bukh. - Ch. 34 Whole Genome Amplification from Single Cells and Minute DNA Samples / Dagan Wells, Mercedes G. Bermudez. - Ch. 35 Primer Extension Preamplification (PEP) of Single Cells: Efficiency and Bias / Xiao Yan Zhong, Ying Li, Wolfgang Holzgreve, Sinuhe Hahn. - Ch. 36 In Vitro Evolution through PCR-Mediated Mutagenesis and Recombination / Sarah B. Faulkner, Heather A. Syrett, Eric A. Davidson, Andrew D. Ellington. - Index.
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Section I : General Considerations. : Template Isolation and Preparation. - Ch. 1 Extraction and Amplification of Ancient DNA / Anne C. Stone. - Ch. 2 Long PCR from Damaged DNA / Bernard Fromenty, Philippe Schmitt, Dominique Pessayre. - Ch. 3 Quantitative mRNA Analysis in Small Cell Samples by RT-PCR and Flow Cytometry / Veronika Stemme, Sten Stemme. - Ch. 4 Microdissection of Single or Small Numbers of Cells for Analyses of DNA and RNA by PCR / Rose G. Mage, Robert F. Bonner, Harold Obiakor. - Ch. 5 Pre-PCR Processing Strategies / Peter Radstrom, Maria Lovenklev, Petra Wolffs, Charlotta Lofstrom, Rickard Knutsson. - Ch. 6 Novel PCR-Enhancing Compounds and Their Modes of Action / Raj Chakrabarti. - Ch. 7 Optimizing PCR with the Aid of Experimental Design / Thomas Weissensteiner. - Ch. 8 Economic Fluorescent Labeling of PCR Products for Microsatellite- and Single-Stranded Conformation Polymorphism (SSCP) Analysis / Markus Schuelke. - Ch. 9 Universal Restriction Site-Free Cloning Method Using Chimeric Primers / Guo Jun Chen. - Ch. 10 High-Throughput Genotyping and the PCR / Kalim U. Mir, Jiannis Ragoussis . - Ch. 11 High-Throughput Genetic Analysis through Multiplexed PCR and Multicapillary Electrophoresis / John M. Butler, Peter M. Vallone. - Ch. 12 The GOOD Assay: A Purification-Free Assay for Genotyping by MALDI Mass Spectrometry / Jorg Tost, Ramon Kucharzak, Doris Lechner, Ivo Glynne Gut. - Ch. 13 Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension (APEX) / Maido Remm, Ants Kurg, Andres Metspalu. - Ch. 14 Surface Plasmon Resonance Based Biosensor Technology for Real-Time Detection of PCR Products / Giordana Feriotto, Roberto Gambari. - Ch. 15 Fluorescent Amplified Fragment Length Polymorphism (FAFLP) Genotyping / Catherine Arnold. - Ch. 16 Global Analysis of DNA Allelic Variations (GADAV) by Specific Enrichment of Mismatches and Selective Amplification of Heterohybrids / Xinghua Pan, Sherman M. Weissman. - Ch. 17 Quantitative DNA-Methylation Analysis by the Bisulfite Conversion Method / Osman El-Maarri, Manfred Kuepper, Johannes Oldenburg, Jorn Walter. - Ch. 18 Pyrosequencing Technology / Pal Nyren, Tommy Nordstrom, Baback Gharizadeh. - Ch. 19 Octamer-Primed Cycle Sequencing / Anelia I. Kraltcheva, Susan H. Hardin. - Ch. 20 In Situ Amplification of cDNA / Gerard J. Nuovo. - Ch. 21 Detection of a Single-Copy Sequence on Human Metaphase by Cyclic PRINS / Kirsten Mennicke, Sven Opitz, Eberhard Schwinger. - Ch. 22 Meaningful Quantification of mRNA Using Real-Time PCR / Stephen A. Bustin. - Ch. 23 Absolute Quantification of Specific Nucleic Acids by RT-PCR Using a Nonlinear Mathematical Model for Data Analysis / Huong L. Vu, Serge Troubetzkoy, Vu Q. Nguyen, Michael W. Russell, Jiri Mestecky. - Ch. 24 Single-Molecule PCR - Basic Protocols and Applications / Paul H. Dear. - Ch. 25 Gene Expression and Discovery: Application of a Genome-Wide Approach / Timothy A. Lohret, Karen M. Kelly. - Ch. 26 The Detection of Differential Gene Expression Changes Using SAGE (Serial Analysis of Gene Expression) / Jill Powell. - Ch. 27 Applications of Differential Display in Infectious Diseases / Irene Bosch, Rajas V. Warke, Marcia F. Fournier, David Magee, Heather Melichar. - Ch. 28 Restriction Endonucleolytic Analysis of Differentially Expressed Sequences: READS / Yerramilli V. B. K. Subrahmanyam, Sherman M. Weissman. - Ch. 29 Suppression Subtractive Hybridization PCR / Wan Ji, Li Cai. - Ch. 30 PCR-Based Techniques for Cloning an Unknown DNA Fragment Adjacent to a Known Integrant / Eric Ka-Wai Hui, Szecheng J. Lo. - Ch. 31 Chromosome Walking by Inverse PCR / Jorge A. Garces, Selwyn A. Williams, Ray H. Gavin. - Ch. 32 Long Universal Fast Walking / Kyl V. Myrick. - Ch. 33 Cloning of Unknown Virus Sequences by DNase Treatment and Sequence-Independent Single Primer Amplification / Tobias Allander, Suzanne U. Emerson, Robert H. Purcell, Jens Bukh. - Ch. 34 Whole Genome Amplification from Single Cells and Minute DNA Samples / Dagan Wells, Mercedes G. Bermudez. - Ch. 35 Primer Extension Preamplification (PEP) of Single Cells: Efficiency and Bias / Xiao Yan Zhong, Ying Li, Wolfgang Holzgreve, Sinuhe Hahn. - Ch. 36 In Vitro Evolution through PCR-Mediated Mutagenesis and Recombination / Sarah B. Faulkner, Heather A. Syrett, Eric A. Davidson, Andrew D. Ellington. - Index.