Primrose, S.B.

Principles of gene manipulation and genomics / S.B. Primrose and R.M. Twyman. - 7th ed. - Malden, MA : Blackwell Pub., c2006. - xxii, 644 p. : ill. ; 28 cm.

Rev. ed. of: Principles of gene manipulation. 6th ed. 2001 and Principles of genome analysis and genomics / Sandy B. Primrose, Richard M. Twyman. 3rd ed. 2003.

Includes bibliographical references (p. [547]-626) and index.

Gene manipulation in the post-genomics era -- Basic techniques -- Cutting and joining DNA molecules -- Basic biology of plasmid and phage vectors -- Cosmids, phasmids, and other advanced vectors -- Gene cloning strategies -- Sequencing genes and short stretches of DNA -- Changing genes : site-directed mutagenesis and protein engineering -- Bioinformatics -- Cloning in bacteria other than Escherichia coli -- Cloning in Saccharomyces cerevisiaea and other fungi -- Gene transfer to animal cells -- Genetic manipulation of animals -- Gene transfer to plants -- Advanced transgenic technology -- The organization and structure of genomes -- Mapping and sequencing genomes -- Comparative genomics -- Large-scale mutagenesis and interference -- Analysis of the transcriptome -- Proteomics I : expression analysis and characterization of proteins -- Proteomics II : analysis of protein structures -- Proteomics III : protein interactions -- Metabolomics and global biochemical networks -- Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci -- Applications of recombinant DNA technology.

1405135441 (pbk.) 9781405135443 (pbk.)


Genetic engineering.
Genomics.
Gene mapping.
Nucleotide sequence.

660.65 / PRI 2006