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Biotechnology : DNA to protein : a laboratory project in molecular biology / Teresa Thiel, Shirley Bissen, Eilene M. Lyons.

By: Thiel, Teresa.
Contributor(s): Bissen, Shirley [(j.a.)] | Lyons, Eilene M [(j.a.)].
Publisher: Boston, Mass. : McGraw-Hill, c2002Description: x, 182 p. : ill. ; 28 cm.ISBN: 0072416645 (pbk.); 0071122796 (pbk.) (Int. ed.).Subject(s): Molecular biology -- Laboratory manuals | Amylases | Biology projects | Molecular cloning -- Laboratory manualsDDC classification: 572.8
Contents:
Preface. - Acknowledgments. - Introduction to the project. - Examine Enzyme Activity of alpha-Amylase. 1. Starch Plate Assay. 2. Quantitative Enzyme Assay. 3. Factors Affecting Enzyme Function. - Examine alpha-Amylase Proteins. 4. Analysis of Protein Structure Using RasMol. 5. Analysis of alpha-Amylase Proteins. 5A. SDS-PAGE. 5B. Western Blotting. - Examine DNA Structure. 6. Analysis of DNA Structure Using RasMol. 7. Isolation of Chromosomal DNA from Bacillus licheniformis. - Find the alpha-Amylase Gene. 8. PCR Amplification and Labeling of Probe DNA. 9. Southern Hybridization. 9A. Restriction Enzyme Cleavage of Chromosomal DNA. 9B. Denaturation and Transfer of DNA to a Membrane. 9C. Southern Hybridization and Detection. - Clone the alpha-Amylase Gene. 10. Cloning the alpha-Amylase Gene. 10A. Cleavage of Chromosomal DNA. 10B. Cleavage of Plasmid DNA. 10C. Ligation of Chromosomal and Plasmid DNA. 10D. Transformation. 10E. Identification of alpha-Amylase Clones. - Analyze alpha-Amylase Clones. 11. Verification and Mapping of alpha-Amylase Clones. 11A. Verification of alpha-Amylase Clones Using PCR. 11B. Isolation of Plasmid DNA from alpha-Amylase Clones. 11C. Restriction Cleavage and Mapping of alpha-Amylase Plasmid DNA. 11D. Preservation of Recombinant Strains. 11E. Southern Analysis of alpha-Amylase Plasmid DNA. 12. Enzyme Activity of alpha-Amylase Clones. - Appendix I. Additional Information and Exercises. - Appendix II. Frequently Used Procedures. - Appendix III. Bibliography.
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Main Collection Taylor's Library-TU

Floor 4, Shelf 16, Side 2, TierNo 3, BayNo 1

 572.8 THI (Browse shelf) 1 Available SBSxx,36100,03,GR 5000081674
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Preface. - Acknowledgments. - Introduction to the project. - Examine Enzyme Activity of alpha-Amylase. 1. Starch Plate Assay. 2. Quantitative Enzyme Assay. 3. Factors Affecting Enzyme Function. - Examine alpha-Amylase Proteins. 4. Analysis of Protein Structure Using RasMol. 5. Analysis of alpha-Amylase Proteins. 5A. SDS-PAGE. 5B. Western Blotting. - Examine DNA Structure. 6. Analysis of DNA Structure Using RasMol. 7. Isolation of Chromosomal DNA from Bacillus licheniformis. - Find the alpha-Amylase Gene. 8. PCR Amplification and Labeling of Probe DNA. 9. Southern Hybridization. 9A. Restriction Enzyme Cleavage of Chromosomal DNA. 9B. Denaturation and Transfer of DNA to a Membrane. 9C. Southern Hybridization and Detection. - Clone the alpha-Amylase Gene. 10. Cloning the alpha-Amylase Gene. 10A. Cleavage of Chromosomal DNA. 10B. Cleavage of Plasmid DNA. 10C. Ligation of Chromosomal and Plasmid DNA. 10D. Transformation. 10E. Identification of alpha-Amylase Clones. - Analyze alpha-Amylase Clones. 11. Verification and Mapping of alpha-Amylase Clones. 11A. Verification of alpha-Amylase Clones Using PCR. 11B. Isolation of Plasmid DNA from alpha-Amylase Clones. 11C. Restriction Cleavage and Mapping of alpha-Amylase Plasmid DNA. 11D. Preservation of Recombinant Strains. 11E. Southern Analysis of alpha-Amylase Plasmid DNA. 12. Enzyme Activity of alpha-Amylase Clones. - Appendix I. Additional Information and Exercises. - Appendix II. Frequently Used Procedures. - Appendix III. Bibliography.