TY  - BOOK
AU  - Sambrook,Joseph
AU  - Russell,David W.
TI  - Molecular cloning: a laboratory manual 
SN  - 0879695765 (hbk.)
U1  - 572.8 
PY  - 2001///
CY  - Cold Spring Harbor, N.Y.
PB  - Cold Spring Harbor Laboratory Press
KW  - Molecular cloning
KW  - Laboratory manuals
KW  - Cloning, Molecular
KW  - Laboratory Manuals
N1  - Includes bibliographical references and index; Preface. - Acknowledgments. - On-line edition. - Quotation credits. - Volume 1. Ch. 1: Plasmids and their usefulness in molecular cloning. - Ch. 2: Bacteriophage and its vectors. - Ch. 3: Working with Bacteriophage M13 vectors. - Ch. 4: Working with high capacity vectors. - Ch. 5: Gel electrophoresis of DNA and pulsed-field Agarose. - Ch. 6: Preparation and analysis of Eukaryotic Genomic DNA. - Ch. 7: Extraction, purification, and analysis of mRNA from Eukaryotic Cells. - Volume 2. Ch. 8: In Vitro Amplification of DNA by the Polymerase Chain reaction. - Ch. 9: Preparation of Radiolabeled DNA and RNA probes. - Ch. 10: Working with Synthetic Oligonucleotide probes. - Ch. 11: Preparation of cDNA libraries and Gene identification. - Ch. 12: DNA sequencing. - Ch. 13: Mutagenesis. - Ch. 14: Screening expression libraries. - Volume 3. Ch. 15: Expression of Cloned Genes in Escherichia coli. - Ch. 16: Introducing Cloned Genes into Cultured Mammalian Cells. - Ch. 17: Analysis of Gene Expression in cultured Mammalian Cells. - Ch. 18: Protein interaction technologies. - Information panels. - Appendices. - Index
ER  -