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Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.

By: Contributor(s): Publication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2001.Edition: 3rd edDescription: 3 v. : ill. ; 28 cmISBN:
  • 0879695765 (hbk.)
Subject(s): DDC classification:
  • 572.8 SAM
Contents:
Preface. - Acknowledgments. - On-line edition. - Quotation credits. - Volume 1. Ch. 1: Plasmids and their usefulness in molecular cloning. - Ch. 2: Bacteriophage and its vectors. - Ch. 3: Working with Bacteriophage M13 vectors. - Ch. 4: Working with high capacity vectors. - Ch. 5: Gel electrophoresis of DNA and pulsed-field Agarose. - Ch. 6: Preparation and analysis of Eukaryotic Genomic DNA. - Ch. 7: Extraction, purification, and analysis of mRNA from Eukaryotic Cells. - Volume 2. Ch. 8: In Vitro Amplification of DNA by the Polymerase Chain reaction. - Ch. 9: Preparation of Radiolabeled DNA and RNA probes. - Ch. 10: Working with Synthetic Oligonucleotide probes. - Ch. 11: Preparation of cDNA libraries and Gene identification. - Ch. 12: DNA sequencing. - Ch. 13: Mutagenesis. - Ch. 14: Screening expression libraries. - Volume 3. Ch. 15: Expression of Cloned Genes in Escherichia coli. - Ch. 16: Introducing Cloned Genes into Cultured Mammalian Cells. - Ch. 17: Analysis of Gene Expression in cultured Mammalian Cells. - Ch. 18: Protein interaction technologies. - Information panels. - Appendices. - Index.
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Includes bibliographical references and index.

Preface. - Acknowledgments. - On-line edition. - Quotation credits. - Volume 1. Ch. 1: Plasmids and their usefulness in molecular cloning. - Ch. 2: Bacteriophage and its vectors. - Ch. 3: Working with Bacteriophage M13 vectors. - Ch. 4: Working with high capacity vectors. - Ch. 5: Gel electrophoresis of DNA and pulsed-field Agarose. - Ch. 6: Preparation and analysis of Eukaryotic Genomic DNA. - Ch. 7: Extraction, purification, and analysis of mRNA from Eukaryotic Cells. - Volume 2. Ch. 8: In Vitro Amplification of DNA by the Polymerase Chain reaction. - Ch. 9: Preparation of Radiolabeled DNA and RNA probes. - Ch. 10: Working with Synthetic Oligonucleotide probes. - Ch. 11: Preparation of cDNA libraries and Gene identification. - Ch. 12: DNA sequencing. - Ch. 13: Mutagenesis. - Ch. 14: Screening expression libraries. - Volume 3. Ch. 15: Expression of Cloned Genes in Escherichia coli. - Ch. 16: Introducing Cloned Genes into Cultured Mammalian Cells. - Ch. 17: Analysis of Gene Expression in cultured Mammalian Cells. - Ch. 18: Protein interaction technologies. - Information panels. - Appendices. - Index.