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008 110218s2008 njua b 001 0 eng
020 _a9781588298171 (hbk.)
020 _a1588298175 (hbk.)
020 _a9781597455831 (ebk.)
020 _a1597455830 (ebk.)
039 9 _a201206111732
_bstazrina
_c201104161042
_dwilmina
_c201103231325
_dizani
_c201102181810
_dVLOAD
_y201102161553
_zmalathy
082 0 4 _a572.815774
_bDRO 2008
245 0 0 _aDrosophila :
_bmethods and protocols /
_cedited by Christian Dahmann.
260 _aTotowa, NJ :
_bHumana Press,
_cc2008.
300 _axi, 437 p. :
_bill. (some col.) ;
_c24 cm.
490 1 _aMethods in molecular biology ;
_v420
504 _aIncludes bibliographical references and index.
505 0 _aDrosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias -- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant -- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium -- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust -- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand -- P-element mutagenesis / Thomas Hummel and Christian Klämbt -- EMS screens : from mutagenesis to screening and mapping / Christian Bökel -- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros -- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic -- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler -- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre -- Immunolabeling of embryos / H.-Arno J. Müller -- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit -- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist -- Immunolabeling of imaginal discs / Thomas Klein -- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.] -- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik -- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause -- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg -- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol -- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz -- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich -- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm -- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar -- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas -- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder.
650 0 _aDrosophila.
_950223
650 0 _aMolecular biology
_xResearch.
_9201827
650 0 _aMolecular biology
_xTechnique.
_9201828
650 0 _aAnimal models in research.
_940005
700 1 _aDahmann, Christian.
_9201829
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_v420.
_947771
920 _aBMS : 160795
999 _c21226
_d21226